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Electroporation System Gemini X2
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Electroporation System Gemini X2
Voltage: 5-3000V
Pulse time: 10 μ s -999 ms
Pulse interval time: 100ms-30s
Introduction:
The Gemini Twin Wave Electroporators are flexible systems allowing both square wave and exponential decay wave electroporation in a single unit. Ideal for all of your electroporation needs, including CRISPR, in vivo, in vitro, in ovo and more. Systems for cuvettes or plates.
These waveform combinations enable researchers to easily and efficiently electroporate eukaryotic and prokaryotic cells in cuvettes or plates with one easy-to-use setup. Prokaryotic cells typically respond well to exponential decay wave pulses and eukaryotic cells are transfected most efficiently with square wave pulses. Combining these two waveforms gives researchers total flexibility to achieve the highest efficiency for their applications.
FEATURES & BENEFITS
-Square wave and exponential decay waves power in a single unit
-Multiple pulsing capabilities in both square and exponential decay waveforms (X2 systems)
-Large 7-inch, easy-to-use touch screen interface
-Universal electroporation – transfects cells in vitro, in vivo, in ovo and adherent forms
-Preset protocols – includes the most common eukaryotic and prokaryotic cell types
-User-defined protocols – unlimited ability to add and modify protocols
-Safety – displays resistance measurements for each pulse with three layers of arc protection
-Data management – stores logs of every pulse delivered for QC and troubleshooting
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Electroporation System Gemini SC
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Electroporation System Gemini SC
Voltage: 5-3000V
Pulse time: 50 μ s -100ms
Pulse interval time: 100ms-30s
Introduction:
The Gemini Twin Wave Electroporators are flexible systems allowing both square wave and exponential decay wave electroporation in a single unit. Ideal for all of your electroporation needs, including CRISPR, in vivo, in vitro, in ovo and more. Systems for cuvettes or plates.
These waveform combinations enable researchers to easily and efficiently electroporate eukaryotic and prokaryotic cells in cuvettes or plates with one easy-to-use setup. Prokaryotic cells typically respond well to exponential decay wave pulses and eukaryotic cells are transfected most efficiently with square wave pulses. Combining these two waveforms gives researchers total flexibility to achieve the highest efficiency for their applications.
FEATURES & BENEFITS
-Square wave and exponential decay waves power in a single unit
-Multiple pulsing capabilities in both square and exponential decay waveforms (X2 systems)
-Large 7-inch, easy-to-use touch screen interface
-Universal electroporation – transfects cells in vitro, in vivo, in ovo and adherent forms
-Preset protocols – includes the most common eukaryotic and prokaryotic cell types
-User-defined protocols – unlimited ability to add and modify protocols
-Safety – displays resistance measurements for each pulse with three layers of arc protection
-Data management – stores logs of every pulse delivered for QC and troubleshooting
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Electroporation System Agile Pulse™ In Vivo
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Electroporation System Agile Pulse™ In Vivo
Voltage: 50-1000V
Pulse time: 5 μ s -10 ms
Pulse interval time: 20 μ s-1s
Introduction:
The AgilePulse In Vivo System, used for animal immunization, vaccine development, and gene therapy, provides an intra-muscular, intra-dermal, or intra-tumor electroporation solution to produce maximum transfection efficiency.
Traditional in vivo nucleic acid delivery systems, such as gene gun delivery, suffer from poor efficiency. The AgilePulse In Vivo System effectively introduces DNA or RNA into the target tissue, representing a powerful and safe means for stimulating an immune response that recognizes and eliminates target molecules in the body.
For vaccine applications, DNA vaccination through the dermal layer is preferred since it is an easily accessible site that is immunologically active. After direct injection of plasmid DNA in the dermal layer, a programmed sequence of electric pulses is applied through a miniature parallel needle electrode array to promote cellular uptake and transfection. Cells in the surrounding tissue are transfected, including dendritic antigen-presenting cells and mesenchymal origin cells. Gene expression simulates the immune system to respond to the secreted antigen. Gene expression in skin is 100-fold higher when delivery is enhanced by electroporation compared to simply injecting plasmid DNA (Roos, et. al. 2009).
Features
-Simple User Interface—touch screen user interface, USB key data storage, and Windows® Mobile 6.0 operating system. The system automatically stores a digitized record of all pulse parameters for quality control. EN 60601-1, EN 60601-1-1 EN 60601-1-2, UL60601-1 and CSA601.1 compliant.
-Pulse Agile® Advantage—patented Pulse Agile technology combines a unique sequence of short, high-intensity pulses to open pores in cell membranes, followed by long, low-intensity pulses to further drive the DNA into cells via electrophoresis. The adjustable pulses improve efficiency while maintaining cell viability. The specialized use of the Pulse Agile waveform has shown to significantly enhance antigen specific CD8+ T-cell response when standard protocols do not.
-Uniform, Reliable Electroporation—AgilePulse parallel-needle arrays produce uniform electric fields across the treatment area for a more thorough transfection of the tissue. Electrode impedance is monitored through software analysis to ensure reliable electrode placement in tissue each and every time.
-Simple, Fast Treatment—simple yet effective intra-dermal or intramuscular electrode design allows for shorter delivery times of less than a second. The electrodes provide a uniform pulse to cover and target larger areas of tissue using a single pulsing application.
-Optimal Design—miniature, 2-mm length needles easily penetrate the layers of skin or muscle and target cells for high efficiency gene delivery.
-Safety—Each needle electrode array comes with a safety cover and easy grip sides to make the electrode insertion procedure simple and safe.
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Electroporation System Agile Pulse™ MAX
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Electroporation System Agile Pulse™ MAX
Voltage: 50-1200V
Pulse time: 5 μ s -10 ms
Pulse interval time: 20 μ s-1s
Introduction:
The AgilePulse MAX System is an advanced electroporation solution for fast, efficient transfection of up to 10 ml of cell suspension. Specifically engineered for large-volume applications, our system maximizes cellular uptake with minimal heating and short cycle-time to ensure high cell viability in further cell processing.
The AgilePulse MAX System is simple to use. Cells and polynucleotide are suspended in our proprietary BTXpress Cytoporation Medium T or Cytoporation Medium T4 and transferred via sterile syringe to the large volume electroporation chamber where a programmed sequence of electric pulses is applied. First, a sequence of short, high-intensity pulses opens pores in the cell membranes. These are followed by long, low-intensity pulses that drive the material into cells via electrophoresis. Our patented PulseAgile technology optimizes these pulse parameters to maximize efficiency and cell viability.
The system includes a user-friendly, programmable waveform generator with patented PulseAgile technology, our patent-pending large-volume electroporation chamber, and proprietary BTXpress Cytoporation Medium T, optimized for large volume electroporation. The system is engineered to provide uniform electric fields in a stable temperature environment for excellent cell viability.
Features
-Scale-up—transfection protocols readily scale-up from standard laboratory cuvettes to large-volume transfection in the AgilePulse MAX system.
-Maximal Efficiency with Cytoporation Medium—BTXpress Cytoporation Medium T used with the AgilePulse MAX system has been optimized for maximal efficiency with a number of cell lines, including K562, A20, HEK293 and CHO-KI. It is compatible with a large range of transfectants including DNA, RNA, siRNA, and olignonucleotides. It can be directly diluted in complete growth medium for post-electroporation cell culture.
-Simple User Interface—all controls are operated with the simple touch screen on the front panel. Data is quickly retrieved by USB key and can be analyzed for detailed pulse characteristics including pulse and pulse current.
-Pulse Agile® Advantage—transfection efficiency and cell viability are enhanced by specialized, programmable electrical pulse waveforms, particularly important for larger polynucleotide delivery such as DNA plasmids. The patented Pulse Agile technology combines a unique sequence of short high-intensity pulses to porate cell membranes, followed by long low-intensity pulses to further drive transfectants into cells via electrophoresis, while maintaining cell viability.
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