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Nucleic acid extraction kit
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Nucleic acid extraction kit
Principle: Nanomagnetic particles
Sample types: blood, serum, urine, nasopharyngeal swabs, cells, feces, tissue specimens, bacterial species, insects, etc
Introduction:
The reagent kit employs superparamagnetic magnetic nanoparticles with high binding affinity as its matrix. In a high-concentration ionizing agent environment, these nanoparticles effectively adsorb nucleic acids through hydrogen bonding and electrostatic attraction, while excluding proteins and other impurities. After adsorption, the nucleic acid-bound nanoparticles undergo a washing step to remove proteins and salts. Finally, the nucleic acid is eluted from the particles using a low-salt buffer. The magnetic beads, featuring specialized surface modifications, exhibit enhanced nucleic acid binding capacity and elution efficiency, thereby significantly reducing the risk of cross-contamination and improving detection sensitivity and accuracy.
This reagent is suitable for isolating high-purity pathogen nucleic acid (DNA/RNA) from a wide range of samples, including liquid specimens like blood, serum, plasma, urine, nasopharyngeal swabs, and cell cultures. Solid samples, such as feces, tissue specimens, bacterial strains, and insects, require preliminary processing, with the use of suspension centrifugation supernatant as an alternative. The extracted nucleic acid can be directly utilized in applications like fluorescence quantitative PCR, biochip analysis, next-generation sequencing, and other related experiments.
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